Reactivity of antibodies to heteroclitic peptides based on the Chlamydia trachomatis major outer-membrane protein
نویسندگان
چکیده
منابع مشابه
Antigenic analysis of the major outer membrane protein of Chlamydia trachomatis with murine monoclonal antibodies.
We prepared monoclonal antibodies against prototype strains of the 15 serovars of Chlamydia trachomatis and identified a subset of reagents that reacted with the major outer membrane protein(s) (MOMPs) of one or more serovars. We then determined the specificities of these anti-MOMP monoclonal antibodies by radioimmunoassay and immunoblot assays against the 15 serovars of C. trachomatis and a C....
متن کاملImmune specificity of murine T-cell lines to the major outer membrane protein of Chlamydia trachomatis.
The antigenically variant Chlamydia trachomatis major outer membrane protein (MOMP) is a target of antibody-mediated neutralization in vitro, and it is an important protein for designing a subunit vaccine. Knowledge of MOMP T-cell determinants will be essential to elicit rapid and strong immune responses following an encounter with infectious organisms. C. trachomatis-specific T-cell lines were...
متن کاملMolecular cloning of the major outer membrane protein of Chlamydia trachomatis.
A gene library of Chlamydia trachomatis (serovar L1) DNA has been prepared in the phage vector lambda 1059. From this bank, 20 recombinant phage-expressing components which reacted with serum from a patient with a C. trachomatis (L1) infection were chosen. Selective expression and radiolabeling of phage polypeptides in irradiated Escherichia coli demonstrated that one of these clones encoded a ...
متن کاملThe major outer membrane protein nucleotide sequence of Chlamydia trachomatis, serovar E.
The major outer membrane protein (MOMP) of C. trachomatis is surface exposed and accounts for 60% of the outer membrane protein of this widespread pathogen. This protein has been shown to contain conserved regions among the 15 serovars of C. trachomatis as well as variable domains (VD) that are partially responsible for antigenic differences among the serovars. In addition antibodies raised to ...
متن کاملNucleotide sequence of the major outer membrane protein gene from Chlamydia trachomatis serovar H.
The gene encoding the major outer membrane protein (MOMP) from Chlamydia trachomatis, serovar H was isolated from a genomic library using the MOMP gene from serovar L2 as the probe (1) . The recombinant insert was subcloned into a plasmid vector (Bluescript), the gene localized by Southern blotting and the nucleotide sequence was determined by the dideoxy chain termination method. The H MOMP ge...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1994
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-140-4-815